A low molecular weight fraction of wheat bran aqueous extract acts as a promoter of fused nanospheres after proteolysis with V8

Protein-rich wheat bran aqueous extract was fractionated by size exclusion chromatography. Each of the chromatographic fractions hydrolyzed with V8 protease in a reaction medium containing Ca2+. Both and hydrolysates were characterized physically chemically. The objective to obtain information about which components are involved formation nanoparticles, reported previous works. Six from 0.3 102.8 kDa obtained. results chemical characterization, electrophoresis, UV-Vis spectroscopy, infrared spectroscopy revealed presence protein-polysaccharide complexes four fractions. Fused nanospheres at different angles found scanning electron microscopy after proteolysis 15.1 fraction. It proposed that such binding could provide sites form branched nanostructures.